| E & B Electrical | Anstruther | Electrician | |
| East Neuk accident Repair Centre | St Monans | Garages | |
| East Neuk Campers | Anstruther | Motoring | |
| East Neuk Cycles | Anstruther | Bicycle Shop | |
| East Neuk Dental Practice | Anstruther | Dentist | |
| East Neuk Garden Centre | Crail | Garden Centres | |
| East Neuk Launderette | Anstruther | Launderette | |
| East Neuk Outdoors | Anstruther | Sports | |
| East Neuk Plumbers & Decorators | Elie | Plumber | |
| East Neuk Shell Fish | Crail | Fish-mongers | |
| East Neuk Veterinary Clinic | Pittenweem | Veterinary | |
| Eccles Architects | Upper Largo | Architects | |
| Edie C & A | Crail | Farms | |
| Electricity Shop | Anstruther | Electrical Shop | |
| Elie Coffee Shop | Elie | Cafes | |
| Elie Golf Professional Shop | Elie | Sports | |
| Elie Harbour Chandlers | Elie | Marine Services | |
| Elie Watersports | Elie | Sports | |
| Elite Secretarial Services | Elie | Secretarial Services | |
| Ellis J & L | Upper Largo | General Stores | |
| Elphick Christine | Upper Largo | Health & Beauty |